SIM1 Antibody

• Catalog Number: orb1248836
• Category: Antibodies
• Description: SIM1 Antibody
• Target: SIM1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SGDRYRTEQYQS
• UniProt ID: P81133
• MW: Approx 80 kDa
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 85.5kDa according to NP_005059.2). Recommended concentration: 1-3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIM1, single-minded homolog 1 (Drosophila), bHLHe1
• Note: For research use only
• NCBI: NP_005059.2

orb1248836 (1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.