SIK2/SNF1LK2 Antibody

SKU: orb1537699

Description

SIK2/SNF1LK2 Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC, IHC-P, IP, WB
• Dilution range: ICC, IF (1:100 - 1:400), IHC, IHC-P (10 µg/ml), IP, WB (1:250)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: LS-B1898 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1898 was determined to be 10 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: S15G10
• Immunogen: Recombinant human SIK2 / SNF1LK2.
• Target: SIK2 / SNF1LK2
• Purification: Affinity purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced from tissue culture supernatant.. Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced from tissue culture supernatant.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

SIK2, KIAA0781, LOH11CR1I, SNF1LK2, QIK, Qin-induced kinase, Salt-inducible kinase 2, SIK-2, SNF1-like kinase 2

Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.

Western blot of extracts from HeLa cells, using anti-SIK2, clone S15G10.

Quick Database Links

Gene Symbol

SIK2 / SNF1LK2