SIK2/SNF1LK2 Antibody

• Catalog Number: orb1537699
• Category: Antibodies
• Description: SIK2/SNF1LK2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S15G10
• Tested applications: ICC, IF, IHC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant human SIK2 / SNF1LK2.
• Concentration: 0.5 mg/ml
• Dilution range: ICC, IF (1:100 - 1:400), IHC, IHC-P (10 µg/ml), IP, WB (1:250)
• Conjugation: Unconjugated
• Target: SIK2 / SNF1LK2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced from tissue culture supernatant.. Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced from tissue culture supernatant.
• Alternative names: SIK2, KIAA0781, LOH11CR1I, SNF1LK2, QIK, Qin-induc
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B1898 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1898 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.

Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.

Western blot of extracts from HeLa cells, using anti-SIK2, clone S15G10.