SIK1 rabbit pAb

• Catalog Number: orb767770
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SIK1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human SIK. AA range:148-197
• MW: 85
• Tested applications: ELISA, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-SIK1 antibody, anti-SIK antibody, anti-SNF1LK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of COLO cells using SIK1 Polyclonal Antibody.

Western blot analysis of lysates from COLO205 cells, treated with PMA 125ng/ml 30', using SIK Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).