SIGLEC8 Antibody (C-term)

• Catalog Number: orb1935016
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2478
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 54042 Da
• Target: This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-406 amino acids from the C-terminal region of human SIGLEC8.
• UniProt ID: Q9NYZ4
• NCBI: NP_055257.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sialic acid-binding Ig-like lectin 8, Siglec-8, Si
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-Siglec8 C-term Pab is used in Western blot to detect Siglec8 in mouse liver tissue lysate (lnae 1) and in HL60 cell lysate (lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.