SIGLEC7 (D-siglec) Antibody (N-term)

• Catalog Number: orb1939539
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2711
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 51143 Da
• Target: This SIGLEC7 (D-siglec) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIGLEC7 (D-siglec).
• UniProt ID: Q9Y286
• NCBI: NP_057627.2, NP_001264130.1, NP_055200.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sialic acid-binding Ig-like lectin 7, Siglec-7, Ad
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Dsiglec-W20 at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: NCI-H460 whole cell lysate. Lane 3: Human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-SIGLEC7 Pab in NCI-H460 cell line lysate (35 ug/lane). SIGLEC7 (arrow) was detected using the purified Pab.