SIGLEC15 Antibody

• Catalog Number: orb1238667
• Category: Antibodies
• Description: SIGLEC15 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-SIGLEC15 antibody (orb1238667) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15. The immunogen is located within amino acids 270-320 of SIGLEC15.
• Concentration: 1 mg/mL
• Dilution range: WB: 2 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 36kDObserved: 34-36kD
• Target: SIGLEC15
• UniProt ID: Q6ZMC9
• NCBI: NP_998767
• Storage: SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SIGLEC15 Antibody: CD33L3, HsT1361, SIGLEC-15, CD3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.