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Catalog Number | orb1934985 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB2487/2488 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 75795 Da |
Target | This SIGLEC11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SIGLEC11. |
UniProt ID | Q96RL6 |
NCBI | NP_443116.2, NP_001128635.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Sialic acid-binding Ig-like lectin 11, Sialic acid Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-SIGLEC11 at 1:500 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-SIGLEC11 at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SIGLEC11 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.