SIAH2 Antibody

• Catalog Number: orb1264032
• Category: Antibodies
• Description: SIAH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat, Xenopus, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SIAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the Central region of human SIAH2.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: SIAH2
• UniProt ID: O43255
• NCBI: O43255
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: E3 ubiquitin-protein ligase SIAH2, 632-, Seven in
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in T47D cell line lysates (35 ug/lane).

SIAH2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.