SHISA2 Polyclonal Antibody

• Catalog Number: orb1417616
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SHISA2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Conjugation: Unconjugated
• UniProt ID: Q9D7I0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein shisa-5, Scotin, Shisa5, Scotin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Muscle (Mouse) Lysate at 40 ug. Primary: Anti-SHISA2 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 31 kD. Observed band size: 31 kD.

Sample: Spleen (Mouse) Lysate at 40 ug. Lymph node (Mouse) Lysate at 40 ug. Primary: Anti-SHISA2 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 31 kD. Observed band size: 31 kD.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SHISA2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.