Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347930
• Category: Antibodies
• Description: Sheep IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in rabbit.
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Sheep IgG alkaline phosphatase Conjuga
• Note: For research use only
• Application notes: Anti-Sheep IgG (H&L) Alkaline Phosphatase has been tested by ELISA and dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit anti-Sheep IgG antibody Alkaline Phosphatase Conjugated. Antigen: Sheep IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng or 2.47 ng as indicated. Primary antibody: N/A. Secondary antibody: Alkaline Phosphatase Conjugated Rabbit anti-Sheep IgG Antibody. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) at RT for 1 hour.