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Sheep IgG F(c) antibody
Catalog Number: orb347913
| Catalog Number | orb347913 |
|---|---|
| Category | Antibodies |
| Description | Sheep IgG F(c) antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Sheep |
| Isotype | IgG |
| Immunogen | Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit. |
| Concentration | 2.0 mg/mL |
| Dilution range | ELISA: 1:10,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab). |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-S Read more... |
| Note | For research use only |
| Application notes | Anti-Sheep IgG F(c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Sheep IgG F(c) Antibody [orb346368]
SDS-PAGE, WB
Unconjugated
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, anti-Sheep IgG and anti-Sheep IgG F(c). No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain.
Sheep
1 mgSheep IgG F(c) Fluorescein Antibody [orb346373]
DOT, IF
FITC
This product was prepared from normal serum by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Sheep IgG, anti-Sheep IgG F(c) and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain.
Sheep
1 mgSheep IgG F(c) Peroxidase Antibody [orb346377]
HRP
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep IgG, anti-Sheep IgG F(c) and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain.
Sheep
1 mgSheep IgG F(c) Biotin Antibody [orb346382]
Biotin
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep IgG, anti-Sheep IgG F(c) and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(ab’)2 or anti- Papain.
Sheep
1 mg
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