SHC1 Antibody

SKU: orb229820

Description

Mouse monoclonal antibody to SHC1

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 2F7C7
• Immunogen: Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli.
• Target: SHC1
• Molecular Weight: 62.8kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SHC; SHCA

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of NIH/3T3 cells using SHC1 antibody

Immunofluorescence analysis of A431 cells using SHC1 antibody

Western blot analysis of human recombinant protein using SHC1 antibody

Western blot analysis of HEK293 using SHC1 antibody

Western blot analysis of NIH/3T3 cell lysate using SHC1 antibody

Quick Database Links

Gene Symbol

SHC1

UniProt

P29353

NCBI Gene ID

6464