SHANK1/SHANK3 Antibody: PerCP

SKU: orb150835

Description

Mouse monoclonal to SHANK1 (PerCP). SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development..

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-460 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N367/51 (Formerly sold as S367-51)
• Immunogen: Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
• Target: SHANK3
• Molecular Weight: 190kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Shank postsynaptic density protein antibody, SH3 and multiple ankyrin repeat domains 3 antibody, Proline rich synapse associated protein 2 antibody, Proline-rich synapse-associated protein 2 antibody, ProSAP2 antibody, PSAP2 antibody, antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody, SH3/ankyrin domain gene 3,Shank3b antibody, SPANK 2 antibody, SPANK2 antibody, KAP/SAPAP interacting protein antibody, OTTHUMP00000174437 antibody, SH3 and multiple ankyrin repeat domains 1 antibody, SH3 and multiple ankyrin repeat domains protein 1 antibody, SH3/ankyrin domain gene 1 antibody, SHANK 1 antibody, Shank1 antibody, Shank1a antibody, Somatostatin receptor interacting protein antibody, Somatostatin receptor-interacting protein antibody, SPANK 1 antibody, SPANK1 antibody, SSTR interacting protein antibody, SSTR-interacting protein antibody, SSTRIP antibody, Synamon antibody, AI841104 antibody, DEL22q13.3 antibody, KIAA1650 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone N367/51. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.

Quick Database Links

Gene Symbol

SHANK3

UniProt

Q9JLU4

NCBI Gene ID

59312

RefSeq (Protein):NP_067708.1