You have no items in your shopping cart.
Cart summary
Item 1 of 1
SHANK1/SHANK3 Antibody: APC
Catalog Number: orb150830
| Catalog Number | orb150830 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to SHANK1 (APC). SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.. |
| Target | SHANK3 |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG2a |
| Conjugation | APC |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Immunogen | Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2. |
| UniProt ID | Q9JLU4 |
| MW | 190kDa |
| Tested applications | ICC, IF, IHC |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Application notes | 1 µg/ml of SMC-460 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Clone Number | N367/51 (Formerly sold as S367-51) |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | Shank postsynaptic density protein antibody, SH3 a Read more... |
| Note | For research use only |
| Entrez | 59312 |
| NCBI | NP_067708.1 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone N367/51. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.
