SH-PTP1 (phospho Tyr536) rabbit pAb

SKU: orb769807

Description

Rabbit polyclonal antibody to SH-PTP1 (phospho-Tyr536)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SHP-1 around the phosphorylation site of Tyr536. AA range:502-551
• Molecular Weight: 67
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PTPN6 antibody, anti-HCP antibody, anti-PTP1C antibody, anti-Tyrosine-protein phosphatase non-receptor type 6 antibody, anti-Hematopoietic cell protein-tyrosine phosphatase antibody, anti-Protein-tyrosine phosphatase 1C antibody, anti-PTP-1C antibody, anti-Protein-tyrosine phosphatase SHP-1 antibody, anti-SH-PT antibody

Western Blot analysis of RAW264.7 + EGF cells using Phospho-SH-PTP1 (Y536) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SHP-1 (Phospho-Tyr536) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5', using SHP-1 (Phospho-Tyr536) Antibody. The lane on the right is blocked with the phospho peptide.