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SGK1/SGK Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IHC-P, WB |
|---|---|
| Dilution Range | ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone No. | 3G8 |
| Immunogen | SGK (AAH01263, 1 aa ~ 91 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Target | SGK1 / SGK |
| Purification | Purified from ascites by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, pH 7.4 |
| Concentration | 0.32 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-SGK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of monoclonal antibody to SGK1 on HeLa cell. [antibody concentration 10 ug/ml].

Detection limit for recombinant GST tagged SGK is approximately 0.1 ng/ml as a capture antibody.
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Protocol Information
SGK1/SGK Antibody (orb1538586)
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