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SGK1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb704332

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb704332
CategoryAntibodies
DescriptionSGK1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number6D1
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human SGK1
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-1000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW55 kDa
TargetSGK1
UniProt IDO00141
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesSerum/glucocorticoid regulated kinase 1; SGK 1; SG
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NoteFor research use only
Expiration Date12 months from date of receipt.
SGK1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SGK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb704332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SGK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb704332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SGK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb704332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SGK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb704332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb704332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Recombinant Rabbit Monoclonal Antibody

Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb704332, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.