SGK1 Mouse Monoclonal Antibody

SKU: orb499779

Description

SGK1 Mouse Monoclonal Antibody

Research Area

Cell Signaling, Intercellular Signaling, Kinases, Molecular Biology, Surface Molecules

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 5E11
• Immunogen: KLH conjugated synthetic peptide derived from human SGK1
• Target: SGK1
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serum/glucocorticoid regulated kinase 1; SGK 1; SGK-1; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase; SGK 1; SGK; SGK1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SGK1) Monoclonal Antibody, Unconjugated (orb499779) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SGK1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SGK1) Monoclonal Antibody, Unconjugated (orb499779) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: Siha (Human) Cell Lysate at 30 ug, Lane 5: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-SGK1 (orb499779) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 45-60 kD, Observed band size: 47 kD.

Quick Database Links

Gene Symbol

SGK1

UniProt

O00141