SFTPA1 Antibody

• Catalog Number: orb1248727
• Category: Antibodies
• Description: SFTPA1 Antibody
• Target: SFTPA1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HLDEELQATLHDFR
• UniProt ID: Q8IWL2
• MW: Approx 30 kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Lung lysates (calculated MW of 27.7kDa according to NP_001087239.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SFTPA1, surfactant protein A1, COLEC4, FLJ50593, F
• Note: For research use only
• NCBI: NP_001087239.2, NP_001092138.1, NP_005402.3, NP_001158118.1, NP_001158117.1

orb1248727 (0.01 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248727 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.