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Anti-SFTPA1/2 Antibody

Catalog Number: orb251573

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb251573
CategoryAntibodies
DescriptionAnti-SFTPA1/2 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, IHC-Fr, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1/2, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW26 kDa
UniProt IDQ8IWL1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPulmonary surfactant-associated protein A2; PSP-A;
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, - Immunofluorescence, 5 μg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-SFTPA1/2 Antibody

IF analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-SFTPA1/2 Antibody

IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in frozen section of Mouse Lung Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-SFTPA1/2 Antibody

IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in frozen section of Rat Lung Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-SFTPA1/2 Antibody

IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-SFTPA1/2 Antibody

IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in paraffin-embedded section of Mouse Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-SFTPA1/2 Antibody

IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in paraffin-embedded section of Rat Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-SFTPA1/2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-SFTPA1/2 Antibody

Western blot analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1/2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1/2 at approximately 26-38 kDa. The expected band size for SFTPA1/2 is at 26 kDa.

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    Unconjugated

    200 μl, 100 μl, 30 μl
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    IF,  IHC,  IHC-Fr,  WB

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