Mouse Pulmonarysurfactant-Associated Protein A1 (SP-A1) ELISA Kit

• Catalog Number: orb1146717
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonarysurfactant-associated protein A1(SP-A1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pulmonarysurfactant-associated protein A1(SP-A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonarysurfactant-associated protein A1(SP-A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pulmonarysurfactant-associated protein A1(SP-A1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Mouse
• Concentration: 20 ng/mL
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: MGC133365, PSAP, PSPA, SFTP1, SFTPA1
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.