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| Catalog Number | orb1146717 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pulmonarysurfactant-associated protein A1(SP-A1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pulmonarysurfactant-associated protein A1(SP-A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pulmonarysurfactant-associated protein A1(SP-A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pulmonarysurfactant-associated protein A1(SP-A1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Concentration | 20 ng/mL |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP-A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SP-A1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.31-20 ng/mL |
| Sensitivity | 0.19 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | MGC133365, PSAP, PSPA, SFTP1, SFTPA1 |
| Note | For research use only |
