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Catalog Number | orb535907 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against SFRP2 conjugated to PerCP |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295) |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); IHC (1:50) |
Conjugation | PerCP |
MW | 37 kDa |
Target | SFRP2 |
Entrez | 6423 |
UniProt ID | Q96HF1 |
NCBI | NP_003004.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | SDF5 antibody, Secreted frizzled-related protein 2 Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-786 was sufficient for detection of SFRP2 in 15 μg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band (s): 37 kDa.