SFRP2 Antibody: Biotin

• Catalog Number: orb535903
• Category: Antibodies
• Description: Rabbit polyclonal antibody against SFRP2 conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:50)
• Conjugation: Biotin
• MW: 37 kDa
• Target: SFRP2
• Entrez: 6423
• UniProt ID: Q96HF1
• NCBI: NP_003004.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: SDF5 antibody, Secreted frizzled-related protein 2
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-786 was sufficient for detection of SFRP2 in 15 μg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band (s): 37 kDa.