SFRP2 Antibody (Biotin)

SKU: orb535903

Description

Rabbit polyclonal antibody against SFRP2 conjugated to Biotin

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000); IHC (1:50)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution of SPC-786 was sufficient for detection of SFRP2 in 15 μg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295)
• Target: SFRP2
• Molecular Weight: 37 kDa
• Purification: Peptide Affinity Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

SDF5, Secreted frizzled-related protein 2, FRP 2, UNQ361/PRO697, sFRP-2, FKSG12, SFRP2_HUMAN, MGC153618, Frizzled related protein 2, MGC53344, FRP2, FRP-2, SARP1, AI851596, SARP 1, MGC128911, SARP-1, Secreted apoptosis related protein 1, ORF Names:, Secreted apoptosis-related protein 1, SDF 5, Sfrp2 protein, sFRP 2, Secreted frizzled related protein 2

Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band (s): 37 kDa.

Quick Database Links

Gene Symbol

SFRP2

UniProt

Q96HF1

NCBI Gene ID

6423

RefSeq (Protein):NP_003004.1