D17Wsu104e Antibody

SKU: orb1272706

Description

D17Wsu104e Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Application Notes:
  ELISA:Indirect:To detect mSF-20 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.SandwichTo detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography employing immobilized mSF-20 matrix.
• Target: D17Wsu104e
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Il25, Ly6elg, Il25, UPF0556 protein C19orf10 homolog, Interleukin-25, IL-25

To detect mSF-20 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SF-20 (orb1272705) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.

To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

D17Wsu104e

UniProt

Q9CPT4

RefSeq:Q9CPT4