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Item 1 of 2
SETDB1 Recombinant Mouse Monoclonal Antibody
Catalog Number: orb1172934
| Catalog Number | orb1172934 |
|---|---|
| Category | Antibodies |
| Description | SETDB1 Recombinant Mouse Monoclonal Antibody |
| Target | SETDB1 |
| Clonality | Recombinant |
| Species/Host | Mouse |
| Isotype | IgG1/Kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Monkey, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | Recombinant human KMT1E protein |
| UniProt ID | Q15047 |
| MW | 143 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-1:1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=0 |
| Antibody Type | Recombinant Antibody |
| Clone Number | H4A4 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ERG-associated protein with SET domain; ESET; H3 K Read more... |
| Note | For research use only |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Lysate: 1: HeLa, 2: JurKat, 3: NIH/3T3, 4: COS-7, 5: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 170 kDa, Observed MW: 170 kDa.
Cell line: Ramos, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172934.
