SETD2 Rabbit pAb Discontinued

SKU: orb136864

Description

Rabbit polyclonal to SETD2

Research Area

Cancer, DNA Damage, Developmental Biology, Epigenetics, Immunology, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2475-2564 of human SETD2 (NP_054878.5).
• Molecular Weight: 288kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SETD2 antibody

Immunofluorescence analysis of C6 cells using SETD2 Rabbit pAb (orb136864) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using SETD2 Rabbit pAb (orb136864) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using SETD2 Rabbit pAb (orb136864) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SETD2 Rabbit pAb (orb136864) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SETD2 Rabbit pAb (orb136864) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SETD2 Rabbit pAb (orb136864) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunoprecipitation analysis of 600 µg extracts of Mouse spleen using 3 µg SETD2 antibody (orb136864). Western blot was performed from the immunoprecipitate using SETD2 antibody (orb136864) at a dilution of 1:1000.

Western blot analysis of lysates from Mouse spleen, using SETD2 Rabbit pAb (orb136864) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Quick Database Links

UniProt

Q9BYW2

Huang, Yaping et al. H3K36me3-mediated mismatch repair preferentially protects actively transcribed genes from mutation J Biol Chem, 293, 7811-7823 (2018)

PubMed: 29610279