SET07 Antibody

• Catalog Number: orb1939440
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 43AT551.86
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IF: 1:10~50, IHC-P: 1:10~50
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 42890 Da
• Target: This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
• UniProt ID: Q9NQR1
• NCBI: NP_065115.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: N-lysine methyltransferase SETD8, 211-, H4-K20-HMT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

(TOP) Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. (Bottom) Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).

All lanes:SET07 Antibody at 1:2000 dilution. Lane 1: 293T whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.