Sestrin-3 Antibody (Center Y335)

• Catalog Number: orb1937442
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18605
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57291 Da
• Target: This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3.
• UniProt ID: P58005
• NCBI: NP_653266.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sestrin-3, SESN3, SEST3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Sestrin-3 Antibody (Center Y335) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).

Sestrin-3 Antibody (Center Y335) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.