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37865 Antibody

SKU: orb1246969

Description

37865 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band seen in Human Brain lysate. Recommended for use at 0.5-2µg/mL.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and (Cerebellum) and Testis. Recommended concentration: 3.75µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: SELVPEPRPKPA-C
Target37865
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SEPT3, septin 3, SEP3, CTA-250D10.3, MGC133218, bK250D10.3, OTTHUMP00000028742, neuronal-specific septin 3, G-Septin
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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

37865 Antibody

orb1246969 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

37865 Antibody

orb1246969 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

37865 Antibody

orb1246969 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

37865 Antibody

orb1246969 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

37865 Antibody (orb1246969)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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