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Catalog Number | orb1254577 |
---|---|
Category | Antibodies |
Description | SEPT7 Antibody |
Target | SEPT7. |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2). |
UniProt ID | Q16181 |
MW | Observed: 51kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, C Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using SEPT7 antibody (orb1254577). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat Intestine using SEPT7 antibody (orb1254577) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody (orb1254577) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody (orb1254577) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SEPT7 antibody (orb1254577).
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |