Separase rabbit pAb Antibody

SKU: orb766298

Description

Rabbit polyclonal antibody to Separase

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816
• Molecular Weight: 230
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ESPL1 antibody, anti-ESP1 antibody, anti-KIAA0165 antibody, anti-Separin antibody, anti-Caspase-like protein ESPL1 antibody, anti-Extra spindle poles-like 1 protein antibody, anti-Separase antibody

Western Blot analysis of various cells using Separase Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of A431 cells using Separase Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

Quick Database Links

UniProt

Q14674