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Catalog Number | orb1259487 |
---|---|
Category | Antibodies |
Description | SEMA4G Antibody |
Target | SEMA4G |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 470-560 of human SEMA4G (NP_001190173.1). |
UniProt ID | Q9NTN9 |
MW | Observed: 105kDa |
Tested applications | WB |
Application notes | WB: 1:200 - 1:2000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | semaphorin-4G, sema domain, immunoglobulin domain Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SEMA4G antibody (orb1259487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
WB | |
Canine, Equine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
FITC |