SEMA4G Antibody

• Catalog Number: orb1259487
• Category: Antibodies
• Description: SEMA4G Antibody
• Target: SEMA4G
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-560 of human SEMA4G (NP_001190173.1).
• UniProt ID: Q9NTN9
• MW: Observed: 105kDa
• Tested applications: WB
• Application notes: WB: 1:200 - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: semaphorin-4G, sema domain, immunoglobulin domain
• Research Area: Neuroscience
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SEMA4G antibody (orb1259487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.