SEMA4C Antibody (C-term)

• Catalog Number: orb1937015
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32649
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:4000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 92623 Da
• Target: This SEMA4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792-821 amino acids of human SEMA4C.
• UniProt ID: Q9C0C4
• NCBI: NP_060259.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Semaphorin-4C, SEMA4C, KIAA1739, SEMAI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SEMA4C Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: U-87MG whole cell lysate. Lane 4: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-SEMA4C Antibody (C-term) at 1:4000 dilution + Neuro-2a whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

SEMA4C Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.