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Catalog Number | orb1934604 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB35876 |
Tested applications | WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 83574 Da |
Target | This SEMA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human SEMA4A. |
UniProt ID | Q9H3S1 |
NCBI | NP_071762.2, NP_001180231.1, NP_001180229.1, NP_001180230.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Semaphorin-4A, Semaphorin-B, Sema B, SEMA4A, SEMAB Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
SEMA4A Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the SEMA4A antibody detected the SEMA4A protein (arrow).
All lanes: Anti-SEMA4A Antibody (N-term) at 1:500 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.