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Securin/PTTG1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IHC-P |
|---|---|
| Dilution range | IF (10 µg/ml), IHC, IHC-P (2.5 - 5 µg/ml), Peptide-ELISA (1:16000) |
| Reactivity | Human, Monkey, Mouse |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-ILDEERELEKLFQ, from the internal region of the protein sequence according to NP_004210.1. |
| Target | Securin / PTTG1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Anti-PTTG1 / Securin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Securin / PTTG1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Securin / PTTG1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton.
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Protocol Information
Securin/PTTG1 Antibody (orb1538489)
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