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secretogranin II Antibody

SKU: orb1248264

Description

secretogranin II Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityCanine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9kDa according to NP_003460.2). Recommended concentration: 0.3-1ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-QELGKLTGPNNQKRE
Targetsecretogranin II
Molecular WeightApprox 70kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

SCG2, secretogranin II, CHGC, EM66, SN, SgII, chromogranin-C, secretogranin-2, secretoneurin

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secretogranin II Antibody

orb1248264 (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

secretogranin II Antibody (orb1248264)

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0.1 mg
$ 880.00
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