SEA0400

SKU: orb1304134

Description

SEA0400 is a potent and selective inhibitor of the Na+/Ca2+ exchanger (NCX). It is widely used in cardiovascular and neurological research to investigate calcium homeostasis in both cellular models and animal studies of ischemia-reperfusion injury and arrhythmia.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 223104-29-8
• MW: 371.38
• Formula: C₂₁H₁₉F₂NO₃
• SMILES: CCOc1ccc(Oc2ccc(OCc3cc(F)ccc3F)cc2)c(N)c1
• Target: Na+/Ca2+ Exchanger|||Calcium Channel
• Solubility: 10% DMSO+90% Corn Oil:2.5 mg/mL (6.73 mM);DMSO:50 mg/mL (134.63 mM)

Bioactivity

• Target IC50:
  NCX:5 -33 nM.
• In Vivo:
  SEA0400, administered intravenously at a dose of 3 mg/kg followed by 3 mg/kg/h for 2 hours, reduces infarct volume in the cerebral cortex and striatum without altering mean regional cortical blood flow in anesthetized rats. Additionally, SEA0400 offers protection against dopaminergic neurotoxicity, as evidenced by maintained dopamine levels in the midbrain and striatum, preserved tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum, normal striatal dopamine release, and avoidance of motor deficits in MPTP-treated C57BL/6J mice.
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• In Vitro:
  SEA0400 inhibits Na+-dependent 45Ca2+ uptake in cultured neurons, astrocytes, and microglia. IC50 values of SEA0400 are 33 nM (neurons), 5.0 nM (astrocytes), and 8.3 nM (microglia). SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation, and production of reactive oxygen species (ROS) in an extracellular Ca2+-dependent manner.
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• Cell Research:
  SEA0400 is dissolved in DMSO (final concentration 0.1%). Cells, plated in 96-well plastic tissue culture plates, are incubated at 37°C for 30 min in normal or Ca2+-free HBSS containing 10 μM H2DCF-DA and 0.25 μg/mL Cremophor EL, and then rinsed twice with normal HBSS to remove excess dye. The cells are reperfused in normal HBSS for 1 h, and the conversion of H2DCF-DA to its fluorescent product dichlorofluorescein by ROS, presumably Water2 and hydroxyl radical, is determined with excitation at 485 nm and emission at 535 nm using a Wallac Multilabel counter. ROS production is expressed as a percentage of control cells. The linearity and sensitivity of ROS assay are confirmed using Water2 prior to the experiment. SEA0400 at the indicated concentrations is added 10 min before Ca2+ reperfusion and present until assay.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ca2+Exchanger, Ca2+ Exchanger, Calcium Channel, CalciumChannel, Inhibitor, Na+Exchanger, Na+/Ca2+ Exchanger, Na+ Exchanger, NCX, inhibit, SEA0400, SEA-0400, SEA 0400

Compound Identifiers

PubChem CID

644100