SEA-0400

SKU: orb1223179

Description

SEA0400 is a Na+/Ca2+ exchanger 1 inhibitor. SEA0400 prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. SEA0400 reduces calcium overload induced by ischemia and reperfusion in mouse ventricular myocytes. SEA0400 attenuates sodium nitroprusside-induced apoptosis in cultured rat microglia. SEA0400, preserves cardiac function and high-energy phosphates against ischemia/reperfusion injury.

Key Properties

• CAS Number: 223104-29-8
• MW: 371.38
• Purity: >98% (HPLC)
• Formula: C₂₁H₁₉F₂NO₃
• SMILES: CCOc1cc(c(cc1)Oc1ccc(cc1)OCc1c(ccc(c1)F)F)N
• Target: NCX
• Solubility: DMSO : ≥ 32 mg/mL; 86.17 mM

Bioactivity

• In Vivo:
  SEA0400 (3 mg/kg + 3 mg/kg/h for 2 h, i. v.) attenuates the infarct volume in the cerebral cortex and striatum, does not affect the mean the regional cortical blood flow in anesthetized rats. SEA0400 protects against the dopaminergic neurotoxicity (determined by dopamine levels in the midbrain and striatum, tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum, striatal dopamine release, and motor deficits) in MPTP-treated C57BL/6J mice.
• In Vitro:
  SEA0400 inhibits Na+-dependent 45Ca2+ uptake in cultured neurons, astrocytes, and microglia. IC50 values of SEA0400 are 33 nM (neurons), 5.0 nM (astrocytes), and 8.3 nM (microglia). SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation, and production of reactive oxygen species (ROS) in an extracellular Ca2+-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SEA0400 | SEA-0400 | SEA 0400

Quick Database Links

Gene Symbol

NCX