SDF1/CXCL12 Antibody

SKU: orb1539011

Description

SDF1/CXCL12 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (2.5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Reactivity: Human
• Application Notes:
  Resuspension: Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human SDF-1alpha (100 ng/ml), a concentration of 2-4 ug/ml of this antibody is required. ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SDF-1alpha as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1alpha. Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

  Read more...

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli derived recombinant Human SDF-1alpha.
• Target: SDF1 / CXCL12
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12

Quick Database Links

Gene Symbol

SDF1 / CXCL12