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Catalog Number | orb428354 |
---|---|
Category | Proteins |
Description | Recombinant of SDF 1a protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The CXCL12 protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 20mM Phosphate buffer pH-7.4 and 150mM NaCl. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK |
Application notes | Chemokines |
Source | Escherichia Coli |
Biological Activity | The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 50-100ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized SDF-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | SDF-1, CXCL12, Pre-B cell growth-stimulating facto Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
11.6 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.5 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.5 kDa | |
E.Coli |