SCUBE2 Antibody (Center N266)

• Catalog Number: orb1938177
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19840
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 109957 Da
• Target: This SCUBE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the Central region of human SCUBE2.
• UniProt ID: Q9NQ36
• NCBI: NP_066025.2, NP_001164161.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Signal peptide, CUB and EGF-like domain-containing
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

SCUBE2 Antibody (Center N266) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 20 ug per lane.