Scicons Mouse double-stranded RNA (K1) Antibody

SKU: orb1972238

Description

Scicons Mouse double-stranded RNA (K1) Antibody

Research Area

Microbiology

Images & Validation

• Tested Applications: ELISA, FC, IAC, IB, ICC, IHC, WB
• Application Notes:
  MAb K1 can be used for ELISA, dsRNA-immunoblotting, immuno-affinity-chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of the antibody for any specific application should be established by titration. Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels. Not for use for clinical purposes. For in vitro use only.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a kappa
• Clone No.: K1
• Purity: Gel electrophoretically pure IgG antibody.
• Purification: Affinity chromatography on Protein A-agarose.
• Conjugation: Unconjugated

Storage & Handling

• Storage: After reconstitution antibodies should be aliquoted and stored at -20 °C or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for 10 years at -20 °C or -70°C.
• Form/Appearance: The lyophilised sample should be reconstituted with 200 µl sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mgr/ml. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend centrifuging (microcentrifuge) the reconstituted antibody before use and using the supernatant.
• Concentration: Concentration after reconstitution: 1.00 mg/ml as determined by A280 nm (A280 nm = 1.47 corresponds to 1 mg/ml antibody).
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse anti dsRNA