SCG2 Antibody

• Catalog Number: orb1257020
• Category: Antibodies
• Description: SCG2 Antibody
• Target: SCG2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-320 of human SCG2 (NP_003460.2).
• UniProt ID: P13521
• MW: Observed: 65kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SCG2, secretogranin II, CHGC, EM66, SN, SgII, chro
• Note: For research use only

Western blot analysis of extracts of various cell lines, using SCG2 antibody (orb1257020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using SCG2 antibody (orb1257020). Blue: DAPI for nuclear staining.