CD14 Antibody (Biotin)

SKU: orb1671422

Description

CD14 Antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human sCD14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14. Western Blot To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
• Target: CD14
• Purification: CD14-specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

soluble CD14, Monocyte differentiation antigen CD14

Quick Database Links

Gene Symbol

CD14

UniProt

P08571

RefSeq:P08571