SCARB2 Antibody

• Catalog Number: orb1246410
• Category: Antibodies
• Description: SCARB2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NKANIQFGDNGTTIS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun. 1992 Apr 30;184(2):604-11.; PMID: 1374238). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SCARB2
• UniProt ID: Q14108
• NCBI: NP_005497.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SCARB2, scavenger receptor class B, member 2, AMRF
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun. 1992 Apr 30;184(2):604-11.; PMID: 1374238). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246410 (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246410 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.