SC35 Antibody

• Catalog Number: orb1731387
• Category: Antibodies
• Description: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide of human SC35
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 25 kDa; Observed MW: 35 kDa
• Entrez: 6427
• UniProt ID: Q01130
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: Protein PR264; Splicing component; 35 kDa; Splicin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.