SBP2 Antibody

• Catalog Number: orb1246981
• Category: Antibodies
• Description: SBP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-KERQERKQRLQEN
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SECISBP2
• UniProt ID: Q96T21
• NCBI: NP_076982.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding prote
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul;26(13):4895-910; PMID: 16782878). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3ug/ml.Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec;36(22):7192-206, PMID: 19004874. Immunoprecipitation: This antibody has also been successfully used for IP.
• Expiration Date: 12 months from date of receipt.

orb1246981 staining of HFF cell lysate with B) 100nM siRNA or A) control siRNA. Detected by chemiluminescence.

orb1246981 (3 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.