SECISBP2 Antibody

SKU: orb1246981

Description

SECISBP2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul;26(13):4895-910; PMID: 16782878). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3µg/mL.Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec;36(22):7192-206, PMID: 19004874. Immunoprecipitation: This antibody has also been successfully used for IP.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KERQERKQRLQEN
• Target: SECISBP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2

orb1246981 staining of HFF cell lysate with B) 100nM siRNA or A) control siRNA. Detected by chemiluminescence.

orb1246981 (3 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Quick Database Links

Gene Symbol

SECISBP2

UniProt

Q96T21

RefSeq (Protein):NP_076982.3