SB225002

SKU: orb1306985

Description

SB225002 is a selective small molecule antagonist of the CXCR2 chemokine receptor, effectively blocking IL-8 binding. It is widely used in research to investigate CXCR2's role in neutrophil migration, inflammation, and cancer biology in both cellular and animal models.

Research Area

Cell Biology, Immunology & Inflammation, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 182498-32-4
• MW: 352.14
• Purity: 98.85%
• Formula: C₁₃H₁₀BrN₃O₄
• SMILES: N(C(NC1=C(Br)C=CC=C1)=O)C2=C(O)C=C(N(=O)=O)C=C2
• Target: CXCR
• Solubility: DMSO:71.4 mg/mL (202.76 mM);Ethanol:3 mg/mL (8.52 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.68 mM)

Bioactivity

• Target IC50:
  CXCR2:22 nM
• In Vivo:
  SB225002 demonstrates antitumor activity as a microtubule inhibitor. It significantly reduce the levels of phosphorylated ERK1/2 and decrease the proliferation of WHCO1 cells. In vitro SB225002 inhibits calcium mobilization stimulated by GROα and effectively suppresse the chemotaxis of human and rabbit neutrophils induced by IL-8 and GROα.
• In Vitro:
  SB225002 demonstrates prolonged analgesic effects and reduces TNBS-induced colitis in mouse models. In rabbits, SB225002 selectively inhibit the margination of neutrophils induced by IL-8. Moreover, at a dosage of 1 mg/kg i.p., SB225002 suppresse the growth of Subcutaneously transplanted tumors in a mouse model of intrahepatic cholangiocarcinoma.
• Cell Research:
  Three esophageal squamous cell carcinoma cell lines WHCO1, WHCO5, and WHCO6 originally established from surgical biopsies of Primary esophageal squamous cell carcinomas are cultured in DMEM containing 10% FCS at 37℃n a humidified atmosphere of 5% CO2. MTT assays are carried out usin the Cell Proliferation kit I. Briefly, 1.5 × 10^3 cells are plated in 96-well plates in a final volume of 180 μL DMEM per well. SB 225002 (antagonist of CXCR2, 400 nM) is added to cells and 0.001% DMSO (solvent) is added as a control. Afte the indicated incubation period, 18 μL o the MTT labeling reagent final concentration 0.5 mg/mL) is added to each well and incubated for 4 hours in a humidified atmosphere. One hundred eighty microliters o the solubilization solution are added to each well an the plates were left overnight at 37℃he spectrophotometric absorbance of Samples is measured at 595 nM using a microtiter plate reader.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, CXCR2, CXCR, CXC chemokine receptors, SB 225002, SB225002, SB-225002

Compound Identifiers

PubChem CID

3854666