SB-3CT

SKU: orb1306757

Description

SB-3CT is a potent, selective inhibitor of gelatinases MMP-2 (Ki=13.9 nM) and MMP-9 (Ki=600 nM). It is widely used in vitro and in vivo to study the role of these enzymes in cancer, inflammation, and neurological disorders.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 292605-14-2
• MW: 306.4
• Purity: 99.38%
• Formula: C₁₅H₁₄O₃S₂
• SMILES: O=S(=O)(CC1CS1)c1ccc(Oc2ccccc2)cc1
• Target: MMP
• Solubility: DMSO:55 mg/mL (179.5 mM);Ethanol:6.1 mg/mL (19.91 mM)

Bioactivity

• Target IC50:
  MMP9:600 nM(Ki)|MMP2:13.9 nM(Ki)
• In Vivo:
  SB-3CT is capable of reducing osteolytic responses, aiding in the maintenance of bone integrity. In PC3 tumor cells, SB-3CT inhibits cellular growth. Within the basement membrane matrix, SB-3CT directly suppresses the infiltration of bone marrow endothelial cells and tubule formation.
• In Vitro:
  In a permanent cerebral ischemia mouse model induced by an embolus, SB-3CT protects neurons from ischemic cell death by inhibiting the degradation of neuronal adhesion proteins. Furthermore, daily intraperitoneal injections of SB-3CT (5-50 mg/kg) in the L-CI.5sT murine lymphoma model significantly hinders liver cancer cell metastasis. Likewise, in a model of osteolytic metastasis of human prostate cancer cells, daily intraperitoneal administration of SB-3CT (50 mg/kg) curtails cell growth, bone resorption, and angiogenesis.
• Cell Research:
  SB-3CT is dissolved in DMSO (10 mM) and stored (-22°C), and then diluted with appropriate media (DMSO 1%) before use. PC3 cells are seeded in 35-mm dishes (5×104 cells/dish) in complete culture medium. The next day, the medium is replaced with complete medium supplemented with 1% DMSO alone (vehicle) or SB-3CT (final concentrations 0.1-50 μM) in 1% DMSO. At various times, the cells are harvested with trypsin and counted.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anticancer, barrier, blood-brain, neuroprotective, MMP, MMP-2, MMP-9, metalloproteinase, inhibit, effects, gelatinases, Inhibitor, Matrix metalloproteinases, matrix, SB 3CT, SB3CT, SB-3CT, permeability

Compound Identifiers

PubChem CID

9883002