SATB2 antibody

SKU: orb2276449

Description

SATB2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells. Negative Control: Colon: SATB2 staining should be absent in stromal and smooth muscle cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-702R
• Immunogen: Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

DNA-binding protein SATB2; GLSS; SATB homeobox 2; Special AT-rich sequence-binding protein 2

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.

Colon Colorectal adenocarcinoma showing strong SATB2 immunostaining of all tumor cells.

Quick Database Links

UniProt

Q9UPW6